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Tissues were lysed by transferring them to pre-filled bead mill tubes (Fisher Scientific, 15-340-154) containing lysis buffer (Tris-HCl 100 mM and 0.5% Triton X-100, pH 8) containing Halt Protease and Phosphatase Inhibitor cocktail and homogenized using a bead mill (VWR) for 10 s

While we did not detect cystathionine by mass spectrometry in these samples, we evaluated the DepMap portal metabolites dataset where cystathionine was quantified across hundreds of cancer cell lines

Methods 25, 402408 (2001)

116 Astrocytic asthenia is also manifested by the limited reactivity in the most vulnerable brain regions such as entorhinal and prefrontal cortices in which astrocytes do not mount a defence response in the presence of -amyloid depositions